Difference between revisions of "Part:BBa K3380660"

 
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<partinfo>BBa_K3380660 short</partinfo>
 
<partinfo>BBa_K3380660 short</partinfo>
  
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Fluorescent RNA aptamer construct flanked by F30 tRNA scaffolds to protect it from degradation. Its transcription is repressed by the ZntR transcription factor. Upon addition of Zinc, the promoter allows the E.coli sigma 70 polymerase to transcribe the construct, resulting in green fluorescence if the DFHBI fluorophore is added.
  
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===Usage and Biology===
 
  
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===Usage and Biology===
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Can be used in cell-free systems as a biosensor.
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 15:02, 27 October 2020


TXO ZntR regulated construct under BBa_K3380105 promoter expressing iSpinach

Fluorescent RNA aptamer construct flanked by F30 tRNA scaffolds to protect it from degradation. Its transcription is repressed by the ZntR transcription factor. Upon addition of Zinc, the promoter allows the E.coli sigma 70 polymerase to transcribe the construct, resulting in green fluorescence if the DFHBI fluorophore is added.


Usage and Biology

Can be used in cell-free systems as a biosensor. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]