Difference between revisions of "Part:BBa K3589206"

 
Line 3: Line 3:
 
<partinfo>BBa_K3589206 short</partinfo>
 
<partinfo>BBa_K3589206 short</partinfo>
  
−
XXXXX
+
<html>
 +
<p>
 +
This composite part contains the PAR-promoter (<a href="https://parts.igem.org/Part:BBa_K3002027">BBa_K3002027</a>) in combination with the RPL23-Terminator (<a href="https://parts.igem.org/Part:BBa_K3002006">BBa_K3002006</a>), cCA secretion signal (<a href="https://parts.igem.org/Part:BBa_K3002007">BBa_K3002007</a>), HA-RGS-8His-tag (<a href="https://parts.igem.org/Part:BBa_K3589151">BBa_K3589151</a>) and the coding sequence of the wildtype laccase from an uncultured marine bacteria (<a href="https://parts.igem.org/Part:BBa_K3589109">BBa_K3589109</a>)  plus the MoClo connectors for positions B1-B2 (<a href="https://parts.igem.org/Part:BBa_K3002302">BBa_K3002302</a>), B2-B3 (<a href="https://parts.igem.org/Part:BBa_K3002303">BBa_K3002303</a>), B4-B5 (<a href="https://parts.igem.org/Part:BBa_K3002304">BBa_K3002304</a>) and B5-B6 (<a href="https://parts.igem.org/Part:BBa_K3002305">BBa_K3002305</a>).
 +
</p>
 +
</html>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 17:21, 25 October 2020


Level 1Wildtype marLac + cCA + HA-RGS-8His

This composite part contains the PAR-promoter (BBa_K3002027) in combination with the RPL23-Terminator (BBa_K3002006), cCA secretion signal (BBa_K3002007), HA-RGS-8His-tag (BBa_K3589151) and the coding sequence of the wildtype laccase from an uncultured marine bacteria (BBa_K3589109) plus the MoClo connectors for positions B1-B2 (BBa_K3002302), B2-B3 (BBa_K3002303), B4-B5 (BBa_K3002304) and B5-B6 (BBa_K3002305).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1379
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 249
    Illegal PstI site found at 1379
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1379
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1379
    Illegal NgoMIV site found at 1239
    Illegal NgoMIV site found at 1958
    Illegal NgoMIV site found at 2409
    Illegal NgoMIV site found at 2929
  • 1000
    COMPATIBLE WITH RFC[1000]