Difference between revisions of "Part:BBa R0080:Experience"

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This promoter is lack of AraC gene so that the promoter can only activate the down-stream expression with the existence of both AraC and Arabinose
  
 
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Revision as of 12:41, 11 October 2014

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_R0080

User Reviews

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Stephanie

The promoter is not inducible by arabinose as it does not contain the required araC gene.

Use I0500 instead which contains the araC gene and the Pbad arabinose controlled promoter.

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iGEM TUDelft 2008, Ostassen

We've used this promoter in our project, and have also found it is always on, independent of arabinose. This result can be found [http://2008.igem.org/Team:TUDelft/Temperature_results#Setting_up_luciferase_measurements here] in figure 3, and has been confirmed on more occasions later.

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