Difference between revisions of "Part:BBa K3468039"
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<partinfo>BBa_K3468039 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3468039 SequenceAndFeatures</partinfo> | ||
Adding hydrogen bonds stabilized α3 helix and α3-β5loop | Adding hydrogen bonds stabilized α3 helix and α3-β5loop | ||
| + | screening, which formed hydrogen bonds with 135-ALA and 136-SER. after mutating it into ARG, it formed hydrogen bonds with 151-THR, which stabilized α3 helix and α3-β5loop, which was beneficial to improve the thermal stability of PETase. | ||
| + | [[File:G139R.png|400px|thumb|left|Fig.1 G139R in PyMOL]] | ||
<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display | ||
Latest revision as of 13:24, 27 October 2020
PETase G139R
The PETase is an enzyme, which can hydrolyze PET and this mutation protein is changed on the basis of the PETase. This protein is changed from G to R at 139 position which can be more stable in higher temperature compared with the wild type.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Adding hydrogen bonds stabilized α3 helix and α3-β5loop screening, which formed hydrogen bonds with 135-ALA and 136-SER. after mutating it into ARG, it formed hydrogen bonds with 151-THR, which stabilized α3 helix and α3-β5loop, which was beneficial to improve the thermal stability of PETase.
