Difference between revisions of "Part:BBa K3468037:Experience"
Line 5: | Line 5: | ||
===Applications of BBa_K3468037=== | ===Applications of BBa_K3468037=== | ||
− | + | The reduction of DDG was predicted by FoldX. PyOML was used for visual screening, which formed hydrogen bonds with 152-ALA itself and ASP-150. after mutating it into SER, it also formed hydrogen bonds with LYS-177, which stabilized α3-β5loop and α4-β6loop, which was beneficial to improve the thermal stability of PETase. | |
[[File:Ala152.png|400px|thumb|left|Fig.1 Ala152 in PyMOL]] | [[File:Ala152.png|400px|thumb|left|Fig.1 Ala152 in PyMOL]] | ||
[[File:A152N.png|400px|thumb|left|Fig.2 A152N in PyMOL]] | [[File:A152N.png|400px|thumb|left|Fig.2 A152N in PyMOL]] |
Revision as of 09:03, 25 October 2020
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K3468037
The reduction of DDG was predicted by FoldX. PyOML was used for visual screening, which formed hydrogen bonds with 152-ALA itself and ASP-150. after mutating it into SER, it also formed hydrogen bonds with LYS-177, which stabilized α3-β5loop and α4-β6loop, which was beneficial to improve the thermal stability of PETase.
User Reviews
UNIQccea936c6ca47d21-partinfo-00000000-QINU UNIQccea936c6ca47d21-partinfo-00000001-QINU