Difference between revisions of "Part:BBa K3606031:Design"
 
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===Design Notes===
 
===Design Notes===
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<p>Part BBa_K3606031 is a new composite part designed to allow CAAP secretion from our engineered E.coli strain by adding a signal peptide novel signal peptide 4 (Nsp4) to the N terminus of CAAP. As a functional peptide that allows surface anchoring of its fused gene product, Nsp4 enables CAAP to be presented on the cellular surface of E.coli. </p>
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<p> The composite part Nsp4_CaAP is a combination of signal peptide Nsp4 and CaAP(Calcium Absorption Peptide). The sequence of Nsp4 is obtained from BBa_K3021004 with no further codon optimization. Then we fused Nsp4(BBa_K3606042) to the N-terminal of CaAP(BBa_K3606000) for its secretion into the extracellular space. </p>
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===Source===
 
===Source===

Latest revision as of 08:37, 25 October 2020


Nsp4-CaAP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 48
    Illegal AgeI site found at 135
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The composite part Nsp4_CaAP is a combination of signal peptide Nsp4 and CaAP(Calcium Absorption Peptide). The sequence of Nsp4 is obtained from BBa_K3021004 with no further codon optimization. Then we fused Nsp4(BBa_K3606042) to the N-terminal of CaAP(BBa_K3606000) for its secretion into the extracellular space.

Source

The sequences of subparts are obtained from E.coli. genome.

References