Difference between revisions of "Part:BBa I0500"
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<partinfo>BBa_I0500 short</partinfo> | <partinfo>BBa_I0500 short</partinfo> | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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When grown with 0.2% arabinose, promoter is weak-medium. [jb, 5/24/04] Part may not be compatible with MC4100 as cell line is araD 139. | When grown with 0.2% arabinose, promoter is weak-medium. [jb, 5/24/04] Part may not be compatible with MC4100 as cell line is araD 139. | ||
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<partinfo>BBa_I0500 SequenceAndFeatures</partinfo> | <partinfo>BBa_I0500 SequenceAndFeatures</partinfo> | ||
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| + | <!-- Uncomment this to enable Functional Parameter display | ||
===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_I0500 parameters</partinfo> | <partinfo>BBa_I0500 parameters</partinfo> | ||
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Revision as of 00:59, 5 June 2006
Inducible pBad/araC promoter
-- No description --
Usage and Biology
When grown with 0.2% arabinose, promoter is weak-medium. [jb, 5/24/04] Part may not be compatible with MC4100 as cell line is araD 139.
PC and AraC are located on the complementary strand, reading right to left as written.
At least one registry stock contains a deletion of the C at base 1194. This is after the transcriptional start but before the translation start, so it may not be significant. Parts with this mutation have been qualitatively observed to function normally.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961