Difference between revisions of "Part:BBa K3429002"
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<partinfo>BBa_K3429002 short</partinfo>
 
<partinfo>BBa_K3429002 short</partinfo>
  
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Flourescence Protein optimized for B. subtilis codon usage.
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Superfolder Green Fluorescent Protein (sfGFP)
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We use this <b>flexible glycine-serine linker</b> (GGGGSGGGGS) to create TasA fusion proteins with CotA and CuO. Huang et al fused TasA to superfolder GFP by using this protein linker without impairing TasA or sfGFP function.
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<a class="anchor" id="cite_note-1"></a>
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The superfolder green fluorescent protein (sfGFP) is a, for B. subtilis codon-optimized, derivative of the GFP. Huang et al. was able to demonstrate its function as a reporter fused with the TasA protein. We implement it as a reporter and experimental control for our TasA fusion proteins with CotA, CuO and EreB.
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             <a class="referencestd" href="https://doi.org/10.1038/s41589-018-0169-2 " target="_blank">7. Huang, J., Liu, S., Zhang, C. et al. Programmable and printable Bacillus subtilis biofilms as engineered living materials. Nat Chem Biol 15, 34–41 (2019) </a>
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<a class="anchor" id="cite_note-1"></a>  
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             <a class="referencestd" href="https://doi.org/10.1038/s41589-018-0169-2 " target="_blank">7. Huang, J., Liu, S., Zhang, C. et al. Programmable and printable Bacillus subtilis biofilms as engineered living materials. Nat Chem Biol 15, 34–41 (2019) </a>  
  
  

Revision as of 06:07, 25 October 2020


Superfolder Green Fluorescent Protein (sfGFP)

Superfolder Green Fluorescent Protein (sfGFP)


The superfolder green fluorescent protein (sfGFP) is a, for B. subtilis codon-optimized, derivative of the GFP. Huang et al. was able to demonstrate its function as a reporter fused with the TasA protein. We implement it as a reporter and experimental control for our TasA fusion proteins with CotA, CuO and EreB.




<a class="anchor" id="cite_note-1"></a> 

           <a class="referencestd" href="https://doi.org/10.1038/s41589-018-0169-2 " target="_blank">7. Huang, J., Liu, S., Zhang, C. et al. Programmable and printable Bacillus subtilis biofilms as engineered living materials. Nat Chem Biol 15, 34–41 (2019) </a>


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]