Difference between revisions of "Part:BBa K3396000"
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<partinfo>BBa_K3396000 short</partinfo>
 
<partinfo>BBa_K3396000 short</partinfo>
  
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The DocS module comes from The C. thermocellum scaffoldin and it could recognize and bind tightly to complementary Coh2 modules.
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The Coch2 module binds DocS (BBa_K3396000) modules constitutively.  
  
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 01:59, 25 October 2020


DocS

The Coch2 module binds DocS (BBa_K3396000) modules constitutively.

Usage and Biology

The DocS[1] module comes from The C. thermocellum scaffoldin and it could recognize and bind tightly to complementary Coh2 modules. The Coh2–DocS pair represents a family of constitutively dimerizing protein pairs. They can be used as a proof-of-concept tool to demonstrate whether a split-protein approach works. These pairs can also be replaced with other inducible protein dimerization pairs for signal controlled purposes.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Reference

[1] BARAK Y, HANDELSMAN T, NAKAR D, et al. Matching fusion protein systems for affinity analysis of two interacting families of proteins: the cohesin-dockerin interaction [J]. J Mol Recognit, 2005, 18(6): 491-501.