Difference between revisions of "Part:BBa K117000:Design"
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==Source of Orgin sequence== | ==Source of Orgin sequence== | ||
− | to be updated | + | to be updated |
===Source=== | ===Source=== | ||
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===References=== | ===References=== | ||
+ | Cloning and Characterization of ColE7 Plasmid. J Gen Microbiol 137:97-100 | ||
+ | |||
+ | Characterization of the cea gene of the ColE7 Plasmid. Mol Gen Genet (1992) 233:177-183 " |
Latest revision as of 16:25, 29 October 2008
Lysis gene (promotes lysis in colicin-producing bacteria strain)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Lysis gene was commercially synthesized by [http://www.sciencewerke.com Science Wereke]
Extraction of Lysis gene/PCR Oligo fragments DNA sequence Oligo fragments with the DNA sequence of 5'-GCAGAATTCGCGGCCGCTTCTAGAG-3' & 5'-ACTAGTAGCGGCCGCTGCAGGCT-3' were used to PCR lysis gene from the blue script cloning vector.
Source of Orgin sequence
to be updated
Source
to be updated
References
Cloning and Characterization of ColE7 Plasmid. J Gen Microbiol 137:97-100
Characterization of the cea gene of the ColE7 Plasmid. Mol Gen Genet (1992) 233:177-183 "