Difference between revisions of "Part:BBa K3440005"

(→‎Characterization)
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Due to the pandemics, we haven’t been able to use biobricks to create the iGEM Stockholm 2020 parts. Those parts were ordered as gene blocks from Integrated DNA Technologies Inc.. As a result, the sequences of the biobricks used are the same, but the scars between biobricks might differ, as well as the final size of the part.
 
Due to the pandemics, we haven’t been able to use biobricks to create the iGEM Stockholm 2020 parts. Those parts were ordered as gene blocks from Integrated DNA Technologies Inc.. As a result, the sequences of the biobricks used are the same, but the scars between biobricks might differ, as well as the final size of the part.
 
We sent this part for sequencing to Microsynth AG. The sequence obtained corresponded to the expected part.  
 
We sent this part for sequencing to Microsynth AG. The sequence obtained corresponded to the expected part.  
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[[File:gelEF.png|thumb|center|500px|Colony PCR gel for BBa_K3440005(E)and BBa_K3440007(F)]]
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[[File:gelEF.png|thumb|center|500px|Colony PCR gel for BBa_K3440004(E)and BBa_K3440005(F)]]
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[[File:T--Stockholm--platef.jpg|thumb|center|500px|Transformation plate for BBa_K3440007(F)]]
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[[File:T--Stockholm--platef.jpg|thumb|center|500px|Transformation plate for BBa_K3440005(F)]]
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 00:34, 25 October 2020


Lux I with myc tag under Promoter prmA activated in the presence of PFOS

Pprma(BBa_K2911000)-RBS(BBa_B0034)-LuxI(BBa_C0061)-Myc(BBa_K823036)

Usage and Biology

Characterization

Due to the pandemics, we haven’t been able to use biobricks to create the iGEM Stockholm 2020 parts. Those parts were ordered as gene blocks from Integrated DNA Technologies Inc.. As a result, the sequences of the biobricks used are the same, but the scars between biobricks might differ, as well as the final size of the part. We sent this part for sequencing to Microsynth AG. The sequence obtained corresponded to the expected part.

Colony PCR gel for BBa_K3440004(E)and BBa_K3440005(F)
Transformation plate for BBa_K3440005(F)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 837
    Illegal BglII site found at 875
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 137
    Illegal NgoMIV site found at 148
  • 1000
    COMPATIBLE WITH RFC[1000]