Difference between revisions of "Part:BBa K3440002"

Revision as of 22:31, 24 October 2020

mCherry under prma promoter

Pprma (BBa_K2911000)-RBS(BBa_B0034)-mCherry (BBa_J06504)-DT(BBa_B0015)

Usage and Biology

Produces mCherry under prma promoter, which can be activated by PFAS/PFOA

Characterization

Due to the pandemics, we haven’t been able to use biobricks to create the iGEM Stockholm 2020 parts. Those parts were ordered as gene blocks from Integrated DNA Technologies Inc.. As a result, the sequences of the biobricks used are the same, but the scars between biobricks might differ, as well as the final size of the part.

Western Blot for BBa_K34440001 (C) and BBa_K3440002 (B)

Fluorescence measurements for BBa_K34440001 (C) and BBa_K3440002 (B)

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 137
Illegal NgoMIV site found at 148
1000
COMPATIBLE WITH RFC[1000]

@@ Line 10: / Line 10: @@
 ===Characterization===
 Due to the pandemics, we haven’t been able to use biobricks to create the iGEM Stockholm 2020 parts. Those parts were ordered as gene blocks from Integrated DNA Technologies Inc.. As a result, the sequences of the biobricks used are the same, but the scars between biobricks might differ, as well as the final size of the part.
+[[File:BBa_K34440001 and BBa_K3440002_WB.png|thumb|center|500px|Western Blot for BBa_K34440001 (C) and BBa_K3440002 (B)]][[File:BBa_K34440001 and BBa_K3440002_Fluo.png|thumb|center|1000px|Fluorescence measurements for BBa_K34440001 (C) and BBa_K3440002 (B)]]
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 <span class='h3bb'>Sequence and Features</span>