Difference between revisions of "Part:BBa K3440003"
(→Usage and Biology) |
(→Characterization) |
||
| Line 11: | Line 11: | ||
Due to the pandemics, we haven’t been able to use biobricks to create the iGEM Stockholm 2020 parts. Those parts were ordered as gene blocks from Integrated DNA Technologies Inc.. As a result, the sequences of the biobricks used are the same, but the scars between biobricks might differ, as well as the final size of the part. | Due to the pandemics, we haven’t been able to use biobricks to create the iGEM Stockholm 2020 parts. Those parts were ordered as gene blocks from Integrated DNA Technologies Inc.. As a result, the sequences of the biobricks used are the same, but the scars between biobricks might differ, as well as the final size of the part. | ||
| − | + | [[File:BBa_K34440001 and BBa_K3440002_WB.png|thumb|center|500px|Colony PCR Gel for BBa_K34440001 (C) and BBa_K3440003 (D)]][[File:leakinessfluo.png|thumb|center|1000px|Fluorescence measurements for BBa_K34440003 (D)]] | |
<!-- --> | <!-- --> | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 22:45, 24 October 2020
GFP under Plux
Plux (BBa_R0062)-RBS(BBa_B0034)-GFP(BBa_E0040)
Usage and Biology
Produces GFP under Plux. Plux can be activated by LuxR:3OC6-HSL.
Characterization
Due to the pandemics, we haven’t been able to use biobricks to create the iGEM Stockholm 2020 parts. Those parts were ordered as gene blocks from Integrated DNA Technologies Inc.. As a result, the sequences of the biobricks used are the same, but the scars between biobricks might differ, as well as the final size of the part.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 725