Difference between revisions of "Part:BBa K3440008"

Revision as of 23:27, 24 October 2020

RhlR with myc tag under constitutive promoter

Pconst(BBa_J23100)-RBS(BBa_B0034)-RhlR(BBa_C0071)-Myc(BBa_K823036)-DT(BBa_B0015)

Usage and Biology

Produces RhlR-Myc under constitutive promoter.

Characterization

Due to the pandemics, we haven’t been able to use biobricks to create the iGEM Stockholm 2020 parts. Those parts were ordered as gene blocks from Integrated DNA Technologies Inc.. As a result, the sequences of the biobricks used are the same, but the scars between biobricks might differ, as well as the final size of the part.

File:PlateJ.png

Transformation plate for J

Colony PCR gel for BBa_K3440007(G) and BBa_K3440008(J)

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 880
Illegal BamHI site found at 301
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 776

@@ Line 13: / Line 13: @@
 ===Characterization===
 Due to the pandemics, we haven’t been able to use biobricks to create the iGEM Stockholm 2020 parts. Those parts were ordered as gene blocks from Integrated DNA Technologies Inc.. As a result, the sequences of the biobricks used are the same, but the scars between biobricks might differ, as well as the final size of the part.
+[[File:plateJ.png|thumb|center|500px|Transformation plate for J]]
+[[File:gelGJ.png|thumb|center|500px|Colony PCR gel for BBa_K3440007(G) and BBa_K3440008(J)]]
 <!-- -->
 <span class='h3bb'>Sequence and Features</span>