Difference between revisions of "Part:BBa K3440016"
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===Usage and Biology===
 
===Usage and Biology===
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This part expresses mtrb constitutively. Mtrb is responsible for electricity production in Shewanella oneidensis.
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This part expresses mtrb constitutively. Mtrb is responsible for electricity production in Shewanella oneidensis. In our project, we created this part as an intermediate to build BBa_K3440019 and BBa_K3440021.  
  
 
===Characterization===
 
===Characterization===
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Due to the pandemics, we haven’t been able to use biobricks to create the iGEM Stockholm 2020 parts. Those parts were ordered as gene blocks from Integrated DNA Technologies Inc.. As a result, the sequences of the biobricks used are the same, but the scars between biobricks might differ, as well as the final size of the part.
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Due to the pandemics, we haven’t been able to use biobricks to create the iGEM Stockholm 2020 parts. Those parts were ordered as gene blocks from Integrated DNA Technologies Inc..  
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Revision as of 19:31, 25 October 2020


Mtrb under constitutive promoter

Pconst(BBa_J23100)-RBS(BBa_B0034)-Mtrb(BBa_K3146001)-Myc(BBa_K823036)

Usage and Biology

This part expresses mtrb constitutively. Mtrb is responsible for electricity production in Shewanella oneidensis. In our project, we created this part as an intermediate to build BBa_K3440019 and BBa_K3440021.

Characterization

Due to the pandemics, we haven’t been able to use biobricks to create the iGEM Stockholm 2020 parts. Those parts were ordered as gene blocks from Integrated DNA Technologies Inc..


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2184
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]