Difference between revisions of "Part:BBa K3440021"

Revision as of 15:00, 24 October 2020

MtrB under oscillatory circuit

Plux(BBa_R0062)-RBS(BBa_B0034)-MtrB(BBa_K3146001)-DT(BBa_B0015)-Pconst(BBa_J23100)-RBS(BBa_B0034)-RhlR(BBa_C0071)-DT(BBa_B0015)-Prhl(BBa_R0071)-RBS(BBa_B0034)-RhlI(BBa_C0070)-RBS(BBa_B0034)-AiiA(BBa_C0060)-RBS(BBa_B0034)-LuxR(BBa_C0062)-DT(BBa_B0015)

Usage and Biology

Due to the leakiness of Prhl, RhlI is expressed and produces C4-HSL. Parallely, RhlR is constitutively expressed. The complex RhlR:C4-HSL induces Prhl. AiiA under Prhl degrades C4-HSL. Thus, with no AHL input, the circuit oscillates.

With a 3OC6-HSL input (produced by LuxI and used as quorum sensing molecule), this part can be used to produce electricity in an oscillatory manner thanks to expression of Mtrb. Indeed, LuxR is also expressed under Prhl and the complex 3OC6-HSL:LuxR activates Plux, the promoter under which Mtrb is placed.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 2324
Illegal NheI site found at 2347
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 4050
Illegal BamHI site found at 2618
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 4181
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 3093

@@ Line 6: / Line 6: @@
 ===Usage and Biology===
-===Characterization===
-Due to the pandemics, we haven’t been able to use biobricks to create the iGEM Stockholm 2020 parts. Those parts were ordered as gene blocks from Integrated DNA Technologies Inc.. As a result, the sequences of the biobricks used are the same, but the scars between biobricks might differ, as well as the final size of the part.
+Due to the leakiness of Prhl, RhlI is expressed and produces C4-HSL. Parallely, RhlR is constitutively expressed. The complex RhlR:C4-HSL induces Prhl. AiiA under Prhl degrades C4-HSL. Thus, with no AHL input, the circuit oscillates.
+With a 3OC6-HSL input (produced by LuxI and used as quorum sensing molecule), this part can be used to produce electricity in an oscillatory manner thanks to expression of Mtrb. Indeed, LuxR is also expressed under Prhl and the complex 3OC6-HSL:LuxR activates Plux, the promoter under which Mtrb is placed.