Difference between revisions of "Part:BBa K3440015"
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RhlI(BBa_C0070)-RBS(BBa_B0034)-AiiA(BBa_C0060) | RhlI(BBa_C0070)-RBS(BBa_B0034)-AiiA(BBa_C0060) | ||
| − | ===Usage | + | ===Usage=== |
| + | This is an intermediate site used in the construction of BBa_K3440020 and BBa_K3440021 | ||
| + | |||
===Characterization=== | ===Characterization=== | ||
Due to the pandemics, we haven’t been able to use biobricks to create the iGEM Stockholm 2020 parts. Those parts were ordered as gene blocks from Integrated DNA Technologies Inc.. As a result, the sequences of the biobricks used are the same, but the scars between biobricks might differ, as well as the final size of the part. | Due to the pandemics, we haven’t been able to use biobricks to create the iGEM Stockholm 2020 parts. Those parts were ordered as gene blocks from Integrated DNA Technologies Inc.. As a result, the sequences of the biobricks used are the same, but the scars between biobricks might differ, as well as the final size of the part. | ||
Revision as of 15:35, 24 October 2020
RhlI-AiiA
RhlI(BBa_C0070)-RBS(BBa_B0034)-AiiA(BBa_C0060)
Usage
This is an intermediate site used in the construction of BBa_K3440020 and BBa_K3440021
Characterization
Due to the pandemics, we haven’t been able to use biobricks to create the iGEM Stockholm 2020 parts. Those parts were ordered as gene blocks from Integrated DNA Technologies Inc.. As a result, the sequences of the biobricks used are the same, but the scars between biobricks might differ, as well as the final size of the part.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 661
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 792
- 1000COMPATIBLE WITH RFC[1000]