Difference between revisions of "Part:BBa K3440008"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| + | Produces RhlR-Myc under constitutive promoter. RhlR can be complexed with C4-HSL and activate Prhl. | ||
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===Characterization=== | ===Characterization=== | ||
Due to the pandemics, we haven’t been able to use biobricks to create the iGEM Stockholm 2020 parts. Those parts were ordered as gene blocks from Integrated DNA Technologies Inc.. As a result, the sequences of the biobricks used are the same, but the scars between biobricks might differ, as well as the final size of the part. | Due to the pandemics, we haven’t been able to use biobricks to create the iGEM Stockholm 2020 parts. Those parts were ordered as gene blocks from Integrated DNA Technologies Inc.. As a result, the sequences of the biobricks used are the same, but the scars between biobricks might differ, as well as the final size of the part. | ||
Revision as of 16:01, 24 October 2020
RhlR with myc tag under constitutive promoter
Pconst(BBa_J23100)-RBS(BBa_B0034)-RhlR(BBa_C0071)-Myc(BBa_K823036)-DT(BBa_B0015)
Usage and Biology
Produces RhlR-Myc under constitutive promoter. RhlR can be complexed with C4-HSL and activate Prhl.
Characterization
Due to the pandemics, we haven’t been able to use biobricks to create the iGEM Stockholm 2020 parts. Those parts were ordered as gene blocks from Integrated DNA Technologies Inc.. As a result, the sequences of the biobricks used are the same, but the scars between biobricks might differ, as well as the final size of the part.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 880
Illegal BamHI site found at 301 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 776