Difference between revisions of "Part:BBa K3440005"
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Due to the pandemics, we haven’t been able to use biobricks to create the iGEM Stockholm 2020 parts. Those parts were ordered as gene blocks from Integrated DNA Technologies Inc.. As a result, the sequences of the biobricks used are the same, but the scars between biobricks might differ, as well as the final size of the part. | Due to the pandemics, we haven’t been able to use biobricks to create the iGEM Stockholm 2020 parts. Those parts were ordered as gene blocks from Integrated DNA Technologies Inc.. As a result, the sequences of the biobricks used are the same, but the scars between biobricks might differ, as well as the final size of the part. | ||
| + | [[File:plateE.png|thumb|center|500px|Transformation plate for BBa_K3440005(E)]] | ||
| + | [[File:gelEF.png|thumb|center|500px|Transformation plate for BBa_K3440005(E)and BBa_K3440007(F)]][[File:leakinessfluo.png|thumb|center|1000px|Fluorescence measurements for BBa_K34440005 (E)]] | ||
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Revision as of 22:51, 24 October 2020
Lux I with myc tag under Promoter prmA activated in the presence of PFOS
Pprma(BBa_K2911000)-RBS(BBa_B0034)-LuxI(BBa_C0061)-Myc(BBa_K823036)
Usage and Biology
Characterization
Due to the pandemics, we haven’t been able to use biobricks to create the iGEM Stockholm 2020 parts. Those parts were ordered as gene blocks from Integrated DNA Technologies Inc.. As a result, the sequences of the biobricks used are the same, but the scars between biobricks might differ, as well as the final size of the part.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 837
Illegal BglII site found at 875 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 137
Illegal NgoMIV site found at 148 - 1000COMPATIBLE WITH RFC[1000]