Difference between revisions of "Part:BBa K2804002"

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==Added by KEYSTONE_A 2020==
 
==Added by KEYSTONE_A 2020==
  
BMP2 and VEGF inhibition promote expression of cartilaginous pathways on sorted skeletal stem-cell (SSC) in vitro.
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BMP2 and VEGF inhibition promote expression of cartilaginous pathways on sorted skeletal stem-cell (SSC) in vitro '''(Figure 3)'''.
  
[[File:KEYSTONE A BMP2 VEGFR effect.jpg|600px|thumb|left|'''Figure: SSC gene expression levels after in vitro treatment of PBS, BMP2, or sVEGFR1.''']]
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Skeletal stem-cells (SSCs) isolated from the articular surface, periosteum and bone marrow all produced higher amounts of cartilage with addition of BMP2 + sVEGFR1 when compared with addition of PBS only '''(Figure 4)'''.
  
 
'''Reference'''
 
'''Reference'''
  
 
Murphy, M. P., Koepke, L. S., Lopez, M. T., Tong, X., Ambrosi, T. H., Gulati, G. S., ... & Steininger, H. (2020). Articular cartilage regeneration by activated skeletal stem cells. Nature Medicine, 1-10.
 
Murphy, M. P., Koepke, L. S., Lopez, M. T., Tong, X., Ambrosi, T. H., Gulati, G. S., ... & Steininger, H. (2020). Articular cartilage regeneration by activated skeletal stem cells. Nature Medicine, 1-10.
 +
 +
[[File:KEYSTONE A BMP2 VEGFR effect.jpg|600px|thumb|left|'''Figure 3. SSC gene expression levels after in vitro treatment of PBS, BMP2, or sVEGFR1.''']]
 +
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[[File:KEYSTONE A BMP2 VEGFR effect_2.jpg|600px|thumb|left|'''Figure 4. Alcian blue staining (for acidic glycans in cartilage) of SSC following chondrogenic differentiation with treatment of PBS or BMP2 + sVEGFR1''']]
  
  

Revision as of 13:59, 24 October 2020


Bone morphogenetic protein 2 (BMP2)

The human bone morphogenetic protein 2 is a bone-growth regulatory factor well known to be a potent osteoinductive protein.

Added by KEYSTONE_A 2020

BMP2 and VEGF inhibition promote expression of cartilaginous pathways on sorted skeletal stem-cell (SSC) in vitro (Figure 3).

Skeletal stem-cells (SSCs) isolated from the articular surface, periosteum and bone marrow all produced higher amounts of cartilage with addition of BMP2 + sVEGFR1 when compared with addition of PBS only (Figure 4).

Reference

Murphy, M. P., Koepke, L. S., Lopez, M. T., Tong, X., Ambrosi, T. H., Gulati, G. S., ... & Steininger, H. (2020). Articular cartilage regeneration by activated skeletal stem cells. Nature Medicine, 1-10.

Figure 3. SSC gene expression levels after in vitro treatment of PBS, BMP2, or sVEGFR1.
Figure 4. Alcian blue staining (for acidic glycans in cartilage) of SSC following chondrogenic differentiation with treatment of PBS or BMP2 + sVEGFR1


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]