Difference between revisions of "Part:BBa K3468047"
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This mutant undergoes proline substitution. Proline has a special ring structure, which can increase the rigidity of the loop, reduce the conformational entropy, and improve the thermal stability of the protein. | This mutant undergoes proline substitution. Proline has a special ring structure, which can increase the rigidity of the loop, reduce the conformational entropy, and improve the thermal stability of the protein. | ||
| + | Maestro website evaluated an result of -1.214，IMUTANT website evaluated a decrease result of -0.32, and an evaluation of FoldX also obtained a thermal stability improvement result of -0.47 kcal/mol. And the result was not ideal. | ||
| + | |||
| + | [[File:S233P.png|400px|thumb|left|Fig.1 S233P in PyMOL]] | ||
| + | |||
| + | [[File:WT S233.png|400px|thumb|left|Fig.1 WT S233 in PyMOL]] | ||
<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display | ||
===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_K3468047 parameters</partinfo> | <partinfo>BBa_K3468047 parameters</partinfo> | ||
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Latest revision as of 13:34, 27 October 2020
PETase S223P
The PETase is an enzyme, which can hydrolyze PET and this mutation protein is changed on the basis of the PETase. This protein is changed from S to P at 223 position which can be more stable in higher temperature compared with the wild type.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
This mutant undergoes proline substitution. Proline has a special ring structure, which can increase the rigidity of the loop, reduce the conformational entropy, and improve the thermal stability of the protein.
Maestro website evaluated an result of -1.214，IMUTANT website evaluated a decrease result of -0.32, and an evaluation of FoldX also obtained a thermal stability improvement result of -0.47 kcal/mol. And the result was not ideal.
