Difference between revisions of "Part:BBa K3599017"
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<partinfo>BBa_K3599017 short</partinfo> | <partinfo>BBa_K3599017 short</partinfo> | ||
− | + | GFP variant that folds robustly even when fused to poorly folded proteins. We used its sequence as a reporting gene to measure the intensity of the expression. | |
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− | + | ===Sequence and Features=== | |
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<partinfo>BBa_K3599017 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3599017 SequenceAndFeatures</partinfo> | ||
− | + | ===Reference=== | |
− | === | + | Pédelacq, J., Cabantous, S., Tran, T. et al. Engineering and characterization of a superfolder green fluorescent protein. <i>Nat Biotechnol</i> 24, 79–88 (2006). [https://doi.org/10.1038/nbt1172 https://doi.org/10.1038/nbt1172] |
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Latest revision as of 06:55, 27 October 2020
superfolder GFP
GFP variant that folds robustly even when fused to poorly folded proteins. We used its sequence as a reporting gene to measure the intensity of the expression.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 13
Reference
Pédelacq, J., Cabantous, S., Tran, T. et al. Engineering and characterization of a superfolder green fluorescent protein. Nat Biotechnol 24, 79–88 (2006). https://doi.org/10.1038/nbt1172