Difference between revisions of "Part:BBa I766557"
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Ste5 scaffold protein; ensures assembly of the protein kinases of the pheromone-activated MAPK cascade into an active complex during mating (Printen & Sprague, 1994) | Ste5 scaffold protein; ensures assembly of the protein kinases of the pheromone-activated MAPK cascade into an active complex during mating (Printen & Sprague, 1994) | ||
− | + | ==Usage and Biology== | |
+ | ===Estonia TUIT Contribution=== | ||
The comparative study on the abundance of the Ste5 scaffold protein depending on the promoter was conducted by Chapman & Asthagiri in 2009 ('''Figure 1'''). As can be seen from Figure 1, STE5 promoter is a very weak promoter, which ensures a lower expression level in comparison even with CYC promoter. | The comparative study on the abundance of the Ste5 scaffold protein depending on the promoter was conducted by Chapman & Asthagiri in 2009 ('''Figure 1'''). As can be seen from Figure 1, STE5 promoter is a very weak promoter, which ensures a lower expression level in comparison even with CYC promoter. | ||
[[File:1(2).jpg|thumb|center|500px|Figure 1. The relative abundance of STE5-MYC-tagged protein expressed from inducible STE5 (induced by α-factor) and constitutive CYC, ADH, TEF, and GPD promoters. The error bars represent SEM (n=3) (Chapman & Asthagiri, 2009). | [[File:1(2).jpg|thumb|center|500px|Figure 1. The relative abundance of STE5-MYC-tagged protein expressed from inducible STE5 (induced by α-factor) and constitutive CYC, ADH, TEF, and GPD promoters. The error bars represent SEM (n=3) (Chapman & Asthagiri, 2009). |
Revision as of 04:47, 24 October 2020
pSte5 (Weak) Promoter
Low expression, constitutive promoter in yeast
Induced by α-factor-pheromone (Printen & Sprague, 1994)
Ste5 scaffold protein; ensures assembly of the protein kinases of the pheromone-activated MAPK cascade into an active complex during mating (Printen & Sprague, 1994)
Usage and Biology
Estonia TUIT Contribution
The comparative study on the abundance of the Ste5 scaffold protein depending on the promoter was conducted by Chapman & Asthagiri in 2009 (Figure 1). As can be seen from Figure 1, STE5 promoter is a very weak promoter, which ensures a lower expression level in comparison even with CYC promoter.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 93
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 93
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 93
- 1000COMPATIBLE WITH RFC[1000]
References
Chapman, S. A., & Asthagiri, A. R. (2009). Quantitative effect of scaffold abundance on signal propagation. Molecular Systems Biology, 5(1), 313. https://doi.org/10.1038/msb.2009.73
Printen, J. A., & Sprague, G. F. (1994). Protein-protein interactions in the yeast pheromone response pathway: Ste5p interacts with all members of the MAP kinase cascade. Genetics, 138(3), 609–619.