Difference between revisions of "Part:BBa K3580102:Design"
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===Design Notes=== | ===Design Notes=== | ||
This part was designed based on the Ptrc-trGPPS-LS(BBa_K3580101)used in the 2013 paper by Alonso-Gutierrez, Jorge et al. The introduction of the one-amino acid mutation reported in the2015 paper by Srividya, Narayanan et al.The one-amino acid mutation was introduced into the limonene synthase of Mentha spicatato accommodate the N345A mutation, as reported in a 2015 paper by Srividya and Narayanan et al.Actually, limonene synthase used in our project was an E. colicodon-optimized version of a truncated sequence, so a mutation was introduced to change asparagine at position 289 to alanine. | This part was designed based on the Ptrc-trGPPS-LS(BBa_K3580101)used in the 2013 paper by Alonso-Gutierrez, Jorge et al. The introduction of the one-amino acid mutation reported in the2015 paper by Srividya, Narayanan et al.The one-amino acid mutation was introduced into the limonene synthase of Mentha spicatato accommodate the N345A mutation, as reported in a 2015 paper by Srividya and Narayanan et al.Actually, limonene synthase used in our project was an E. colicodon-optimized version of a truncated sequence, so a mutation was introduced to change asparagine at position 289 to alanine. | ||
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===Source=== | ===Source=== | ||
Revision as of 06:13, 24 October 2020
Ptrc-trGPPS-SS(Sabinene synthase)
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1980
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 1980
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2366
Illegal XhoI site found at 4022 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1980
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1980
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part was designed based on the Ptrc-trGPPS-LS(BBa_K3580101)used in the 2013 paper by Alonso-Gutierrez, Jorge et al. The introduction of the one-amino acid mutation reported in the2015 paper by Srividya, Narayanan et al.The one-amino acid mutation was introduced into the limonene synthase of Mentha spicatato accommodate the N345A mutation, as reported in a 2015 paper by Srividya and Narayanan et al.Actually, limonene synthase used in our project was an E. colicodon-optimized version of a truncated sequence, so a mutation was introduced to change asparagine at position 289 to alanine.
Source
Improved for BBa_K3052001so that it can synthesize sabinene and be used in combination with an addgenene plasmid (pBbA5c-MevT-MBI) encoding a series of enzymes in the mevalonate pathway. The products of the mevalonate pathway (IPP and DMAPP) can also be synthesized by the non-mevalonate pathway. This part has improved modularity in the sense that it can use both the mevalonate and non-mevalonate pathways for upstream metabolic pathways.