Difference between revisions of "Part:BBa K3365000:Design"
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===Source=== | ===Source=== | ||
| − | The sequence of the part is from the plasmid, pWJ66. | + | The sequence of the part is from the plasmid, pWJ66. The fragment is synthesized by the company. |
===References=== | ===References=== | ||
Revision as of 04:53, 24 October 2020
dCas9-ω
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1340
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1340
Illegal NheI site found at 1099 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1340
Illegal BamHI site found at 3378
Illegal BamHI site found at 4212 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1340
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1340
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
None.
Source
The sequence of the part is from the plasmid, pWJ66. The fragment is synthesized by the company.
References
[1] BIKARD D, JIANG W, SAMAI P, et al. Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system[J]. Nucleic Acids Res, 2013,41(15): 7429-7437. [2] Yao R, Liu D, Jia X, Zheng Y, Liu W, Xiao Y. CRISPR-Cas9/Cas12a biotechnology and application in bacteria. Synth Syst Biotechnol. 2018 Oct 3;3(3):135-149.