Difference between revisions of "Part:BBa K3365000"
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===Usage and Biology===
 
===Usage and Biology===
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The first plasmid part xxxx is generated by adding the dCas9 with a constitutive promoter to the plasmid vector pACYC184 which has chloramphenicol resistance marker and low copy number. The second plasmid part yyyy contains the sgRNA construction module, the target-RFP sequence and the ampicillin resistance marker from high-copy-number vector pUC57.
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Revision as of 03:08, 24 October 2020


dCas9-ω

RpoZ, an RNAP ω subunit which can activate transcription by recruiting the RNAP holoenzyme is fused to dCas9 C-terminal to activate gene transcription downstream of the protospacer.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1340
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1340
    Illegal NheI site found at 1099
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1340
    Illegal BamHI site found at 3378
    Illegal BamHI site found at 4212
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1340
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1340
  • 1000
    COMPATIBLE WITH RFC[1000]