Difference between revisions of "Part:BBa K3697000"
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This is the coding sequence for producing a codon-optimized mCherry in B. subtilis. mCherry is a derivative of RFP. mCherry_BSU was created by codon-optimizing an E. coli mCherry for expression B. subtilis. When paired with a strong RBS and constitutive promotor (BBa_K3697010), mCherry_BSU can be quantified using a plate reader, and transformed B. subtilis colonies can be moderately red to the naked eye. | This is the coding sequence for producing a codon-optimized mCherry in B. subtilis. mCherry is a derivative of RFP. mCherry_BSU was created by codon-optimizing an E. coli mCherry for expression B. subtilis. When paired with a strong RBS and constitutive promotor (BBa_K3697010), mCherry_BSU can be quantified using a plate reader, and transformed B. subtilis colonies can be moderately red to the naked eye. | ||
| − | mCherry_BSU has an excitation peak at 585 nm and a peak emission at 615 nm. | + | mCherry_BSU has an excitation peak at 585 nm and a peak emission at 615 nm. Color is readily visible in E. coli and B. subtilis in under 12 hours of incubation. |
| − | Fluorescence levels in B. subtilis and E. coli can be quantified using a fluorescent plate reader. Under high enough expression from a strong constitutive promotor such as pVeg (BBa_K143012), red colonies may be visible to the naked eye. E. coli expressing mCherry_BSU are red under natural light. | + | Fluorescence levels in B. subtilis and E. coli can be quantified using a fluorescent plate reader. Under high enough expression from a strong constitutive promotor such as pVeg (BBa_K143012), red colonies may be visible to the naked eye. E. coli expressing mCherry_BSU are red under natural light. E. coli liquid cultures may appear red to the naked eye, while B. subtilis liquid cultures likely will not appear red. |
This protein can be used as a reporter in B. subtilis due to it's high visibility. | This protein can be used as a reporter in B. subtilis due to it's high visibility. | ||
Revision as of 00:20, 24 October 2020
mCherry BSU
This is the coding sequence for producing a codon-optimized mCherry in B. subtilis. mCherry is a derivative of RFP. mCherry_BSU was created by codon-optimizing an E. coli mCherry for expression B. subtilis. When paired with a strong RBS and constitutive promotor (BBa_K3697010), mCherry_BSU can be quantified using a plate reader, and transformed B. subtilis colonies can be moderately red to the naked eye.
mCherry_BSU has an excitation peak at 585 nm and a peak emission at 615 nm. Color is readily visible in E. coli and B. subtilis in under 12 hours of incubation.
Fluorescence levels in B. subtilis and E. coli can be quantified using a fluorescent plate reader. Under high enough expression from a strong constitutive promotor such as pVeg (BBa_K143012), red colonies may be visible to the naked eye. E. coli expressing mCherry_BSU are red under natural light. E. coli liquid cultures may appear red to the naked eye, while B. subtilis liquid cultures likely will not appear red.
This protein can be used as a reporter in B. subtilis due to it's high visibility.
This part does not have a degradation tag.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 4
Illegal AgeI site found at 715 - 1000COMPATIBLE WITH RFC[1000]