Difference between revisions of "Part:BBa K3389003"
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This part codes for an expression system for the RAD27 flap endonuclease 1 ("flappase") from Saccharomyces cerevisiae, including a 10x His tag for purification purposes. RAD27, also known as FEN-1, is a structure-specific flap endonuclease that will cleave overlapping oligonucleotides at the site of a single target nucleotide in the context of a known sequence. One use for this is to distinguish between single nucleotide polymorphisms. The expression system has a T7 promoter, a strong RBS, and a 10x His tag. | This part codes for an expression system for the RAD27 flap endonuclease 1 ("flappase") from Saccharomyces cerevisiae, including a 10x His tag for purification purposes. RAD27, also known as FEN-1, is a structure-specific flap endonuclease that will cleave overlapping oligonucleotides at the site of a single target nucleotide in the context of a known sequence. One use for this is to distinguish between single nucleotide polymorphisms. The expression system has a T7 promoter, a strong RBS, and a 10x His tag. | ||
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+ | ===Characterization=== | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 02:01, 17 October 2021
RAD27 Flappase expression construct with 10x His tag
This part codes for an expression system for the RAD27 flap endonuclease 1 ("flappase") from Saccharomyces cerevisiae, including a 10x His tag for purification purposes. RAD27, also known as FEN-1, is a structure-specific flap endonuclease that will cleave overlapping oligonucleotides at the site of a single target nucleotide in the context of a known sequence. One use for this is to distinguish between single nucleotide polymorphisms. The expression system has a T7 promoter, a strong RBS, and a 10x His tag.
Characterization
Add more about this part here
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 311
Illegal BglII site found at 704 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 216