Difference between revisions of "Part:BBa K3360004"
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| + | The traditional fraying process is very harmful to the environment, resources and users. In essence, being old is the destruction of cellulose on the surface of jeans. We finally consider using cellulase to achieve this effect. Through cellulase washing, it can hydrolyze the cellulose and make a part of the dye fall off the fabric. Through this effect, the "worn feeling" effect that people want can be obtained. | ||
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| + | We chose the CL34 endoglucanase from Streptomyces. We loaded CL34 into pet-28a vector as shown below. Under the induction of IPTG, CL34 will be expressed in large quantities. | ||
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<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display | ||
Revision as of 21:30, 23 October 2020
endo-beta-1_4-glucanase from Streptomyces
Encoding the endo-beta-1,4-glucanase of Streptomyces.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 431
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
SZU-China 2020 TEAM
The traditional fraying process is very harmful to the environment, resources and users. In essence, being old is the destruction of cellulose on the surface of jeans. We finally consider using cellulase to achieve this effect. Through cellulase washing, it can hydrolyze the cellulose and make a part of the dye fall off the fabric. Through this effect, the "worn feeling" effect that people want can be obtained.
We chose the CL34 endoglucanase from Streptomyces. We loaded CL34 into pet-28a vector as shown below. Under the induction of IPTG, CL34 will be expressed in large quantities.