Difference between revisions of "Part:BBa K3589109:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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The natural secretion signal of Botrytis aclada was removed (?). This part is codon-optimized for Escherichia coli.
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The natural secretion signal of <I>Botrytis aclada</I> was removed. This part is codon-optimized for <I>Escherichia coli</I>.
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+
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===Source===
 
===Source===

Revision as of 01:26, 28 October 2020


Wildtype laccase from uncultured marine bacteria for Chlamydomonas reinhardtii (Phytobrick)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 817
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 817
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 817
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 817
    Illegal NgoMIV site found at 677
    Illegal NgoMIV site found at 1396
    Illegal NgoMIV site found at 1847
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The natural secretion signal of Botrytis aclada was removed. This part is codon-optimized for Escherichia coli.

Source

This part originates from Botrytis aclada (Scheiblbrandner, S., 2017) and is condon-optimized for Escherichia coli.

References