Difference between revisions of "Part:BBa K3606077"
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<partinfo>BBa_K3606077 short</partinfo> | <partinfo>BBa_K3606077 short</partinfo> | ||
− | + | <h2>Usage and Biology:</h2> | |
+ | This part completely integrates our quorum sensing system. LuxPR driven tetR is our "switch" for quorum sensing, and P9 driven mcbABCD and PtetR driven mcbEFG are our MccB17 synthesis and secretion system to respond to luxPR driven tetR. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 00:38, 28 October 2020
P9 driven mcbABCD; PtetR driven mcbEFG; luxPR driven tetR
Usage and Biology:
This part completely integrates our quorum sensing system. LuxPR driven tetR is our "switch" for quorum sensing, and P9 driven mcbABCD and PtetR driven mcbEFG are our MccB17 synthesis and secretion system to respond to luxPR driven tetR.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 5547
Illegal XbaI site found at 5532
Illegal XbaI site found at 6856
Illegal SpeI site found at 5960
Illegal PstI site found at 2370
Illegal PstI site found at 2403
Illegal PstI site found at 5946 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 5547
Illegal NheI site found at 6918
Illegal SpeI site found at 5960
Illegal PstI site found at 2370
Illegal PstI site found at 2403
Illegal PstI site found at 5946
Illegal NotI site found at 5539
Illegal NotI site found at 5951 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 5547
Illegal BamHI site found at 3366
Illegal XhoI site found at 5697 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 5547
Illegal XbaI site found at 5532
Illegal XbaI site found at 6856
Illegal SpeI site found at 5960
Illegal PstI site found at 2370
Illegal PstI site found at 2403
Illegal PstI site found at 5946 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 5547
Illegal XbaI site found at 5532
Illegal XbaI site found at 6856
Illegal SpeI site found at 5960
Illegal PstI site found at 2370
Illegal PstI site found at 2403
Illegal PstI site found at 5946
Illegal NgoMIV site found at 2032
Illegal AgeI site found at 2204 - 1000COMPATIBLE WITH RFC[1000]