Difference between revisions of "Part:BBa K3338013:Design"

 
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===References===
 
===References===
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Potorac, I., Rivero-Müller, A., Trehan, A., Kiełbus, M., Jozwiak, K., Pralong, F., Hafidi, A., Thiry, A., Ménagé, J. J., Huhtaniemi, I., Beckers, A., & Daly, A. F. (2016). A vital region for human glycoprotein hormone trafficking revealed by an LHB mutation. <i>The Journal of endocrinology</i>, 231(3), 197–207.

Latest revision as of 17:21, 23 October 2020


CMV-EGFP-P2A-mCherry


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None


Source

CMV and EGFP are part of the pEGFP-C2 vector used for cloning. P2A originally originated from the porcine teschovirus 2A. The sequence of mCherry was purchased from Addgene (plasmid #45350) (Potorac et al. 2016).

References

Potorac, I., Rivero-Müller, A., Trehan, A., Kiełbus, M., Jozwiak, K., Pralong, F., Hafidi, A., Thiry, A., Ménagé, J. J., Huhtaniemi, I., Beckers, A., & Daly, A. F. (2016). A vital region for human glycoprotein hormone trafficking revealed by an LHB mutation. The Journal of endocrinology, 231(3), 197–207.