Difference between revisions of "Part:BBa K3606031"
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K3606031 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3606031 SequenceAndFeatures</partinfo> | ||
| − | + | <p>The secretion of CAAP(Calcium Absorption Peptide) after its expression is indispensable for it to display the robust function of combining calcium. Here, we decided to achieve CAAP secretion via the type II secretion system, which generally contains two steps. The first step involves some signal peptides targeted to the Sec secretory pathways, which are responsible for transporting CAAP across the inner membrane into the periplasm. For Gram-negative bacteria like E.coli, the second step can then take place once CAAP is in the periplasm, as the intrinsic protein complex GspD creates a pore in the outer cell membrane through which CAAP can be secreted out of the bacteria. </p> | |
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Revision as of 08:10, 25 October 2020
Nsp4-CaAP
We fused NSP4 to the N-terminal of CaAP for its secretion into the extracellular space.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 48
Illegal AgeI site found at 135 - 1000COMPATIBLE WITH RFC[1000]
The secretion of CAAP(Calcium Absorption Peptide) after its expression is indispensable for it to display the robust function of combining calcium. Here, we decided to achieve CAAP secretion via the type II secretion system, which generally contains two steps. The first step involves some signal peptides targeted to the Sec secretory pathways, which are responsible for transporting CAAP across the inner membrane into the periplasm. For Gram-negative bacteria like E.coli, the second step can then take place once CAAP is in the periplasm, as the intrinsic protein complex GspD creates a pore in the outer cell membrane through which CAAP can be secreted out of the bacteria.