Difference between revisions of "Part:BBa K3594006"
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[[File:T--SHSBNU China--SHSBNU China parts33.jpeg|500px|thumb|center|The Degradation Pathway of Guaiacol]] | [[File:T--SHSBNU China--SHSBNU China parts33.jpeg|500px|thumb|center|The Degradation Pathway of Guaiacol]] | ||
Ferredoxin Reductase is used as a redox chaperone protein in the process of generating catechol through demethylation, catalyzed by cytochrome P450 (CYP).In an article from AMB Express in 2019, the authors did a very detailed study on the demethylation process of guaiacol. Their data shows that only two enzymes from the G+ bacteria Rhodococcus rhodochrous need to be prepared, namely Cytochrome P450 (WP_085469912 from R. rhodochrous J3) and Ferredoxin reductase (WP_085469913 from R. rhodochrous J3), and transfer them to G-bacteria Pseudomonas After putida EM42, with or without additional carbon source supplementation, the effective degradation of guaiacol can be achieved. | Ferredoxin Reductase is used as a redox chaperone protein in the process of generating catechol through demethylation, catalyzed by cytochrome P450 (CYP).In an article from AMB Express in 2019, the authors did a very detailed study on the demethylation process of guaiacol. Their data shows that only two enzymes from the G+ bacteria Rhodococcus rhodochrous need to be prepared, namely Cytochrome P450 (WP_085469912 from R. rhodochrous J3) and Ferredoxin reductase (WP_085469913 from R. rhodochrous J3), and transfer them to G-bacteria Pseudomonas After putida EM42, with or without additional carbon source supplementation, the effective degradation of guaiacol can be achieved. | ||
| + | |||
| + | <b>References:</b> | ||
| + | J. García‑Hidalgo et al., AMB Express. 9 (34) (2019) | ||
| + | <u>https://amb-express.springeropen.com/articles/10.1186/s13568-019-0759-8#Tab1</u> | ||
==Characterization== | ==Characterization== | ||
Latest revision as of 16:12, 23 October 2020
Ferredoxin Reductase(FR)
Usage and Biology
Ferredoxin Reductase is used as a redox chaperone protein in the process of generating catechol through demethylation, catalyzed by cytochrome P450 (CYP).In an article from AMB Express in 2019, the authors did a very detailed study on the demethylation process of guaiacol. Their data shows that only two enzymes from the G+ bacteria Rhodococcus rhodochrous need to be prepared, namely Cytochrome P450 (WP_085469912 from R. rhodochrous J3) and Ferredoxin reductase (WP_085469913 from R. rhodochrous J3), and transfer them to G-bacteria Pseudomonas After putida EM42, with or without additional carbon source supplementation, the effective degradation of guaiacol can be achieved.
References: J. García‑Hidalgo et al., AMB Express. 9 (34) (2019) https://amb-express.springeropen.com/articles/10.1186/s13568-019-0759-8#Tab1
Characterization
we assume that the change of protein concentration in the external environment is related to the amount of gene expression, the rate of bacterial lysis, the rate of protein degradation, and the rate of locust intestinal peristalsis. [File:T--SHSBNU China--protein concentration-gene expression.png|500px|thumb|center|protein concentration]]
The concentration of the protein changes over time. The abscissa represents time, and the ordinate represents the protein concentration. It can be seen from the image that the protein increases rapidly after the bacterial self-lysis. After reaching a certain concentration, due to the degradation rate of the protein itself and the locust intestinal peristalsis. The rate decreases slightly, and the concentration increases with the next bacterial self-lysis, which forms a stable cycle that can release enzymes efficiently.
The predicted concentration of guaiacol accord with our goal.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 394
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 34
Illegal NgoMIV site found at 355
Illegal NgoMIV site found at 406
Illegal NgoMIV site found at 841
Illegal NgoMIV site found at 1000 - 1000COMPATIBLE WITH RFC[1000]