Difference between revisions of "Part:BBa K3351005"
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===Reference=== | ===Reference=== |
Revision as of 07:59, 25 October 2020
HD5d5, an antimicrobial peptide.
Summary
A human antimicrobial peptide with 32 amino acids.A simplified derivative of HD5 constructed by disulfide reduction and Arg introduction, is able to bind to and penetrate MDRAb membranes, causing cell death by catastrophic collapse and metabolic disruption. In addition to the contribution to electrostatic interactions, Arg also benefits peptides by inducing negative Gaussian curvature and pore formation on bacterial membranes. And HD5d5 enters cytoplasm, where it interacts with cellular contents and decreased the activities of SOD and catalase, resulting in an accumulation of ROS in the cells. Due to the correlation with metalloenzyme inactivation and DNA destruction, the overload of ROS is responsible for the elimination of E. coli and A. baumannii by many antibiotics.
Characterization
Reference
[1] Wang C, Zhao G, Wang S, Chen Y, Gong Y, Chen S, Xu Y, Hu M, Wang X, Zeng H, Wang A, Liu D, Su Y, Cheng T, Chen F, Wang J. A Simplified Derivative of Human Defensin 5 with Potent and Efficient Activity against Multidrug-Resistant Acinetobacter baumannii. Antimicrob Agents Chemother. 2018 Jan 25;62(2):e01504-17. doi: 10.1128/AAC.01504-17. PMID: 29158275; PMCID: PMC5786806.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]