Difference between revisions of "Part:BBa K3338011:Design"
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===Source=== | ===Source=== | ||
| − | The CMV-Promoter and EGFP-Reporter were contained in pEGFP-C2. MagA originates from <i>Magnetospirillum magneticum</i>, P2A from the porcine teschovirus 2A and hGLuc is a human codon optimized form of <i>Gaussia< | + | The CMV-Promoter and EGFP-Reporter were contained in pEGFP-C2. MagA originates from <i>Magnetospirillum magneticum</i>, P2A from the porcine teschovirus 2A and hGLuc is a human codon optimized form of <i>Gaussia</i> luciferase from <i>Gaussia princeps</i>. |
===References=== | ===References=== | ||
Latest revision as of 18:53, 23 October 2020
CMV-EGFP-MagA-P2A-hGLuc
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2553
Illegal BsaI.rc site found at 1885
Illegal BsaI.rc site found at 2434
Illegal SapI site found at 1575
Design Notes
The individual proteins in the EGFP-MagA-P2A-hGLuc-cassette have to be in frame. EGFP was used as a reporter for MagA expression and localization because in order to test transfection efficiency and the correct function of the P2A peptide, MRI detection is to slow and costly.
Source
The CMV-Promoter and EGFP-Reporter were contained in pEGFP-C2. MagA originates from Magnetospirillum magneticum, P2A from the porcine teschovirus 2A and hGLuc is a human codon optimized form of Gaussia luciferase from Gaussia princeps.