Difference between revisions of "Part:BBa K3468016:Design"
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===Design Notes=== | ===Design Notes=== | ||
By observing and comparing the structure of PETase, appropriate mutations were constructed and the forces between the residues were modified. By finding suitable sites around M128, L131, and V156 for mutations, the hydrophobic interaction between the residues was enhanced and the hydrophobic core was created, so Q127L was designed. | By observing and comparing the structure of PETase, appropriate mutations were constructed and the forces between the residues were modified. By finding suitable sites around M128, L131, and V156 for mutations, the hydrophobic interaction between the residues was enhanced and the hydrophobic core was created, so Q127L was designed. | ||
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| + | [[File:Q127L1.png|400px|]] | ||
===Source=== | ===Source=== | ||
Revision as of 14:31, 24 October 2020
PETase Q127L
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
By observing and comparing the structure of PETase, appropriate mutations were constructed and the forces between the residues were modified. By finding suitable sites around M128, L131, and V156 for mutations, the hydrophobic interaction between the residues was enhanced and the hydrophobic core was created, so Q127L was designed.
Source
Ideonalla sakaiensis