Difference between revisions of "Part:BBa K3468078"
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There is new salt bridge and hydrogen bond increased between N37D and R267. Salt bridge is a kind of electric interaction. | There is new salt bridge and hydrogen bond increased between N37D and R267. Salt bridge is a kind of electric interaction. | ||
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| + | We use FoldX to judge the thermal stability of N37D and Jmol to find whether there is a new salt bridge. Finally we use Pymol to find out whether there is other interactions to make it become more stable. | ||
| + | [[File:N37D.png|400px|thumb|left|Fig.1 N37D in PyMOL]] | ||
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===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_K3468078 parameters</partinfo> | <partinfo>BBa_K3468078 parameters</partinfo> | ||
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Latest revision as of 14:04, 27 October 2020
PETase N37D
The PETase is an enzyme, which can hydrolyze PET and this mutation protein is changed on the basis of the PETase. This protein is changed from N to D at 37 position which can be more stable in higher temperature compared with the wild type.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 9
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
There is new salt bridge and hydrogen bond increased between N37D and R267. Salt bridge is a kind of electric interaction.
We use FoldX to judge the thermal stability of N37D and Jmol to find whether there is a new salt bridge. Finally we use Pymol to find out whether there is other interactions to make it become more stable.
