Difference between revisions of "Part:BBa K3468075"
 
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There is new salt bridge and hydrogen bond increased between A40D and K252. Salt bridge is a kind of electric interaction.
 
There is new salt bridge and hydrogen bond increased between A40D and K252. Salt bridge is a kind of electric interaction.
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We use FoldX to judge the thermal stability of A40D and Jmol to find whether there is a new salt bridge. Finally we use Pymol to find out whether there is other interactions to make it become more stable.
 +
[[File:A40D.png|400px|thumb|left|Fig.1 A40D in PyMOL]]
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===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K3468075 parameters</partinfo>
 
<partinfo>BBa_K3468075 parameters</partinfo>
 
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Latest revision as of 14:02, 27 October 2020


PETase A40D

The PETase is an enzyme, which can hydrolyze PET and this mutation protein is changed on the basis of the PETase. This protein is changed from A to D at 40 position which can be more stable in higher temperature compared with the wild type.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

There is new salt bridge and hydrogen bond increased between A40D and K252. Salt bridge is a kind of electric interaction. We use FoldX to judge the thermal stability of A40D and Jmol to find whether there is a new salt bridge. Finally we use Pymol to find out whether there is other interactions to make it become more stable.

Fig.1 A40D in PyMOL