Difference between revisions of "Part:BBa K3468074"
 
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This mutant increases a salt bridge between E280 and R285. Salt bridge is a kind of electric interaction.  
 
This mutant increases a salt bridge between E280 and R285. Salt bridge is a kind of electric interaction.  
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We use FoldX to judge the thermal stability of D220E and Jmol to find whether there is a new salt bridge.
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[[File:D220E.png|400px|thumb|left|Fig.1 D220E in PyMOL]]
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===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K3468074 parameters</partinfo>
 
<partinfo>BBa_K3468074 parameters</partinfo>
 
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Latest revision as of 14:01, 27 October 2020


PETase D220E

The PETase is an enzyme, which can hydrolyze PET and this mutation protein is changed on the basis of the PETase. This protein is changed from D to E at 220 position which can be more stable in higher temperature compared with the wild type.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

This mutant increases a salt bridge between E280 and R285. Salt bridge is a kind of electric interaction. We use FoldX to judge the thermal stability of D220E and Jmol to find whether there is a new salt bridge.

Fig.1 D220E in PyMOL