Difference between revisions of "Part:BBa K3365061:Design"
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===Design Notes=== | ===Design Notes=== | ||
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===Source=== | ===Source=== | ||
| − | + | We constructe this part by PCR and then add homologous region to the fragment by performing another PCR for assemble fragments onto plasmids by Gibson Assembly. | |
===References=== | ===References=== | ||
Latest revision as of 12:24, 26 October 2020
Target transcription activating unit upstream of RFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 66
Illegal NheI site found at 89 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 812
Illegal XhoI site found at 803 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 667
Illegal AgeI site found at 779 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Source
We constructe this part by PCR and then add homologous region to the fragment by performing another PCR for assemble fragments onto plasmids by Gibson Assembly.