Difference between revisions of "Part:BBa K3589107:Design"
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===Source=== | ===Source=== | ||
− | This part originates from | + | This part originates from <I>Botrytis aclada</I> (Yang, Q., 2018) and is condon-optimized for Chlamydomonas reinhardtii. |
===References=== | ===References=== |
Revision as of 01:18, 28 October 2020
Mutant laccase from Botrytis aclada for Chlamydomonas reinhardtii, Part 1 (Phytobrick)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 608
Illegal PstI site found at 695
Illegal PstI site found at 759
Illegal PstI site found at 1131
Illegal PstI site found at 1419 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 608
Illegal PstI site found at 695
Illegal PstI site found at 759
Illegal PstI site found at 1131
Illegal PstI site found at 1419 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1055
Illegal BamHI site found at 1394 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 608
Illegal PstI site found at 695
Illegal PstI site found at 759
Illegal PstI site found at 1131
Illegal PstI site found at 1419 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 608
Illegal PstI site found at 695
Illegal PstI site found at 759
Illegal PstI site found at 1131
Illegal PstI site found at 1419 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The natural secretion signal of the marine bacteria was removed (?). This part is codon-optimized for Chlamydomonas reinhardtii. Additionally, it is compatible with Phytobricks /MoClo parts.
Source
This part originates from Botrytis aclada (Yang, Q., 2018) and is condon-optimized for Chlamydomonas reinhardtii.