Difference between revisions of "Part:BBa K165037:Design"
Line 16: Line 16:
 
Mumberg, Muller, and Funk:
 
Mumberg, Muller, and Funk:
 
http://dx.doi.org/10.1016/0378-1119(95)00037-7
 
http://dx.doi.org/10.1016/0378-1119(95)00037-7
 +
 +
Here are the primers we used:
 +
 +
''example: spacer + biobrick prefeix + homologous to yeast genome''
 +
 +
TEF.fwd:  GTTTCTT + attacccataaggttgtttgtgacgg + agcgttggttggtggatcaag
 +
 +
''example: homologous to yeast genome + biobrick suffix + spacer''
 +
 +
TEF.rev:  cggtcaacgaactataattaacta + tactagtagcggccgctgcag + AAGAAAC
  
 
===References===
 
===References===

Revision as of 09:03, 29 October 2008

TEF2 yeast constitutive promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

-


Source

Amplified from the yeast genome (strain W303a) using promoter definition from:

Mumberg, Muller, and Funk: http://dx.doi.org/10.1016/0378-1119(95)00037-7

Here are the primers we used:

example: spacer + biobrick prefeix + homologous to yeast genome

TEF.fwd: GTTTCTT + attacccataaggttgtttgtgacgg + agcgttggttggtggatcaag

example: homologous to yeast genome + biobrick suffix + spacer

TEF.rev: cggtcaacgaactataattaacta + tactagtagcggccgctgcag + AAGAAAC

References