Difference between revisions of "Part:BBa K3573002:Experience"

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===Applications of BBa_K3573002===
 
===Applications of BBa_K3573002===
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Korea_HS team have performed binding assays using size-exclusion chromatography. We ran three separate runs of 3 superdex75 10/300GL column with buffer (20mM Tris-HCl pH 7.0+250mM NaCl)
 
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1. scFv(RAS) only
 
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2. HRAS only
 
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3. scFv(RAS)+HRAS
 
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The result showed that HRAS's elution peak shifted to higher molecular weight position in the presence of scFv(RAS) compared to HRAS only, indicating their binding.
 
  
 
===User Reviews===
 
===User Reviews===

Revision as of 00:02, 26 October 2020


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Please enter how you used this part and how it worked out.

Applications of BBa_K3573002

User Reviews

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