Difference between revisions of "Part:BBa K3365009"

Revision as of 19:10, 27 October 2020

Lure3 sequence downstream of pBAD

The PAM and potential off-target sequence are located directly downstream of the promoter (BBa_K3365003), where dCas9 might bind wrongly and block RNAP. In our part, the “lure3” is the potential off-target sequence for PDCD1 CRISPR gene editing predicted by our off-target predictor software built in 2019, which might be identified and bound by the complex of dCas9 and sgRNA. According to the off-target predictor software, the relative off-target rate is 73.6673%.

Figure1. Gene circuit

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 74
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 56

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3365009 short</partinfo>
-Lure3 sequence downstream of pBAD...
+The PAM and potential off-target sequence are located directly downstream of the promoter (BBa_K3365003), where dCas9 might bind wrongly and block RNAP. In our part, the “lure3” is the potential off-target sequence for <i> PDCD1</i> CRISPR gene editing predicted by our off-target predictor software built in 2019, which might be identified and bound by the complex of dCas9 and sgRNA. According to the off-target predictor software, the relative off-target rate is 73.6673%.
+<center>[[File:BBa_K3365009.png]]</center>
+<center><b>Figure1.</b> Gene circuit</center>
 <!-- Add more about the biology of this part here