Difference between revisions of "Part:BBa K3463018:Experience"
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how you used this part and how it worked out. | how you used this part and how it worked out. | ||
| − | === | + | ===Characterization of BBa_K3463018=== |
| + | To validate the UTR designer prediction and check the B0017 relevance, we measured the fluorescence and the absorbance of the construction PtacHigh-GFP, PtacLow-GFP, PtacHigh-GFP-B0017, PtacLow-GFP-B0017, and a non-transformed strain as a negative control for to 4-5h | ||
| + | |||
| + | [[Image:T--Grenoble Alpes--Res Survival Ptac construction.png|600px|thumb|center| '''Figure 1''' Effect of UTR prediction and B0017 terminator on eGFP fluorescence expression. | ||
| + | The Figure 5 shows that no fluorescence was detected in the culture of the control non- | ||
| + | transformed strain. As predicted by UTR designer the strong 5’UTR sequence (high) gave higher fluorescent levels than the weak 5’UTR sequence (Low). The terminator B0017 seems to have a limited effect on the fluorescent levels. The last seems to only impact the eGFP production of the strong UTR sequence.]] | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 16:07, 26 October 2020
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how you used this part and how it worked out.
Characterization of BBa_K3463018
To validate the UTR designer prediction and check the B0017 relevance, we measured the fluorescence and the absorbance of the construction PtacHigh-GFP, PtacLow-GFP, PtacHigh-GFP-B0017, PtacLow-GFP-B0017, and a non-transformed strain as a negative control for to 4-5h
Figure 1 Effect of UTR prediction and B0017 terminator on eGFP fluorescence expression. The Figure 5 shows that no fluorescence was detected in the culture of the control non- transformed strain. As predicted by UTR designer the strong 5’UTR sequence (high) gave higher fluorescent levels than the weak 5’UTR sequence (Low). The terminator B0017 seems to have a limited effect on the fluorescent levels. The last seems to only impact the eGFP production of the strong UTR sequence.
User Reviews
UNIQe730c78b91cfeb90-partinfo-00000000-QINU UNIQe730c78b91cfeb90-partinfo-00000001-QINU