Difference between revisions of "Part:BBa K3463018"

 
 
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<partinfo>BBa_K3463018 short</partinfo>
 
<partinfo>BBa_K3463018 short</partinfo>
  
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The design of this sequence was based on the Sang Woo Seo et al. 2012 article to modulate the expression of the following genes. This is a RBS for the eGFP reporter gene (BBa_K1094400) with a low translation score of 1019402,78. This RBS will be used coupled to the eGFP reporter gene, with high expression, as a control for the E7 construction (Composite part name).
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this composite part is composed by the Ptac promoter, a UTR sequence, the reporter gene that codes for GFP and the terminator B0017. The purpose of this composite part is to get a proof of concept by GFP for our survival genetic network (J23-RhlR → Prhlr-LacI → Ptac-GFP) and to assay our in-silico UTR designer prediction.
  
  

Latest revision as of 16:05, 26 October 2020


Ptac RBS high GFP B0017

this composite part is composed by the Ptac promoter, a UTR sequence, the reporter gene that codes for GFP and the terminator B0017. The purpose of this composite part is to get a proof of concept by GFP for our survival genetic network (J23-RhlR → Prhlr-LacI → Ptac-GFP) and to assay our in-silico UTR designer prediction.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]