Difference between revisions of "Part:BBa K117002:Design"
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===Design Notes=== | ===Design Notes=== | ||
LsrA is initially commercially synthesized with RBS BBa_B0034 by ATIbiotech in a blue script cloning vector. LsrA is then extracted using PCR with oligo fragments of DNA sequence 5'-GAATTATGTATACATAATGGCA-3' & 5'-GGTAAATATCCTTTAATTAAA-3' with Biobricks prefix and suffix respectively. | LsrA is initially commercially synthesized with RBS BBa_B0034 by ATIbiotech in a blue script cloning vector. LsrA is then extracted using PCR with oligo fragments of DNA sequence 5'-GAATTATGTATACATAATGGCA-3' & 5'-GGTAAATATCCTTTAATTAAA-3' with Biobricks prefix and suffix respectively. | ||
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==Source of Origin Sequence== | ==Source of Origin Sequence== | ||
Molecular cloning of a gene encoding the thermoactive levansucrase from Rahnella aquatilis and its growth phase-dependent expression in Escherichia coli Jeong-Woo Seo, Ki-Bang Song, Ki-Hyo Jang, Chul-Ho Kim, Bong-Hyun Jung, Sang-Ki Rhee * Microbial and Bioprocess Engineering Laboratory, Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon 305 -600, South Korea | Molecular cloning of a gene encoding the thermoactive levansucrase from Rahnella aquatilis and its growth phase-dependent expression in Escherichia coli Jeong-Woo Seo, Ki-Bang Song, Ki-Hyo Jang, Chul-Ho Kim, Bong-Hyun Jung, Sang-Ki Rhee * Microbial and Bioprocess Engineering Laboratory, Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon 305 -600, South Korea | ||
Revision as of 04:56, 29 October 2008
LsrA promoter (indirectly activated by AI-2)
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
LsrA is initially commercially synthesized with RBS BBa_B0034 by ATIbiotech in a blue script cloning vector. LsrA is then extracted using PCR with oligo fragments of DNA sequence 5'-GAATTATGTATACATAATGGCA-3' & 5'-GGTAAATATCCTTTAATTAAA-3' with Biobricks prefix and suffix respectively.
Source of Origin Sequence
Molecular cloning of a gene encoding the thermoactive levansucrase from Rahnella aquatilis and its growth phase-dependent expression in Escherichia coli Jeong-Woo Seo, Ki-Bang Song, Ki-Hyo Jang, Chul-Ho Kim, Bong-Hyun Jung, Sang-Ki Rhee * Microbial and Bioprocess Engineering Laboratory, Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon 305 -600, South Korea
Pitfalls
Wild type K12 E coli contains LsrA
References
Molecular cloning of a gene encoding the thermoactive levansucrase from Rahnella aquatilis and its growth phase-dependent expression in Escherichia coli Jeong-Woo Seo, Ki-Bang Song, Ki-Hyo Jang, Chul-Ho Kim, Bong-Hyun Jung, Sang-Ki Rhee *Microbial and Bioprocess Engineering Laboratory, Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon 305 -600, South Korea
Regulation of Uptake and Processing of the Quorum-Sensing Autoinducer AI-2 in Escherichia coli Karina B. Xavier and Bonnie L. Bassler* Department of Molecular Biology, Princeton University, Princeton, New Jersey