Difference between revisions of "Part:BBa K3482020"
(→‎Usage and Biology)
 
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===Usage and Biology===
 
===Usage and Biology===
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The CcdA protein gives an immunity against the CcdB toxin by strong complex CcdA-CcdB and therefore prenvent the CcdB toxin to interact with the DNA gyrase.  
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The CcdA protein gives immunity against the CcdB toxin by forming a strong complex CcdA-CcdB and therefore preventing the CcdB toxin to interact with the DNA gyrase.  
  
 
This part is used to create a kill-switch in combination with the part <html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K3482016"> BBa_K3482016 </a></html>.
 
This part is used to create a kill-switch in combination with the part <html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K3482016"> BBa_K3482016 </a></html>.

Latest revision as of 17:02, 26 October 2020


aTc-inducible ccdA antitoxin

CcdA antitoxin under a pTet promoter

Usage and Biology

The CcdA protein gives immunity against the CcdB toxin by forming a strong complex CcdA-CcdB and therefore preventing the CcdB toxin to interact with the DNA gyrase.

This part is used to create a kill-switch in combination with the part BBa_K3482016 .

Characterization

All the data concerning the characterization of that part can be found in the composite part : BBa_K3482016 .


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]