Difference between revisions of "Part:BBa K3365055"

 
 
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<partinfo>BBa_K3365055 short</partinfo>
 
<partinfo>BBa_K3365055 short</partinfo>
  
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This part is used to detect whether dCas9 protein is off-target. The sequence contains lure sequences,which is the potential off-target site for PDCD1-targeted dCas9, and J23117 sperated by 36 base pairs.  
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This part is used to detect whether dCas9 protein is off-target. The sequence contains lure sequences,which is the potential off-target site for PDCD1-targeted dCas9, and J23117 seperated by 36 base pairs.  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 12:33, 27 October 2020


First potential off-target site for dCas9-ω

This part is used to detect whether dCas9 protein is off-target. The sequence contains lure sequences,which is the potential off-target site for PDCD1-targeted dCas9, and J23117 seperated by 36 base pairs.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 66
    Illegal NheI site found at 89
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]